| Solution Information | help | |
| Enzyme: | Neuropeptide Y receptor type 1 | |
| inhibitor: | BDBM61825 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine whether compounds identified as active in a previous set of experiments entitled, "Primary cell-based high-throughput screening assay for potentiators or agonists of NPY-Y2" (AID 1359), "Confirmation cell-based high-throughput screening assay for potentiators or agonists of NPY-Y2" (AID 1539), and inactive in a set of experiments entitled, "Primary cell-based high-throughput screening assay for potentiators or agonists of NPY-Y1" (AID 1304), and "Fluorescence-based counterscreen assay for potentiators or agonists of NPY-Y2: cell-based high-throughput screening assay to identify inhibitors of cyclic nucleotide gated ion channel (CNGC) activity" (PubChem AID 1703), were nonselective due to activation of NPY-Y1. In this assay, a cell line transfected with Y1 and a cyclic-nucleotide gated channel (CNGC) is used to measure potentiation of the EC10 NPY response or direct agonism by test compound of the Y1 receptor. The cells are | |
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